Em. Products sold or licensed by CST Cy3. compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or Excitation. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed However, total fl uorescence of Alexa Fluor 555 conjugates is higher (Figure 5). Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying Description: Alexa Fluor 555 bound to goat anti-mouse IgG antibody Phalloidin conjugates should be reconstituted in pure methanol to make stock solutions, please refer to the directions for use for details. In addition, is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM. We offer Alexa Fluor 555 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. However, Alexa Fluor® 488 tends to be brighter and more optimal for intracellular applications. DRAQ5 is a registered trademark of Biostatus Limited. Select dye-labeling chemistries for conjugation with the following reactive groups: Select an optimized labeling kit for your target antibody or protein: Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Brighter and more photostable when conjugated to antibodies. F-actin was labeled with green-fluorescent Invitrogen Alexa Fluor 488 phalloidin, and the nucleus was stained with Invitrogen TO-PRO 3 iodide (pseudocolored magenta). We offer a broad range of Alexa Fluor 555 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more: See all Alexa Fluor 555 Primary Antibodies See all Alexa Fluor 555 Secondary Antibodies. Stock solutions are stable for 1 year at -20°C, desiccated and protected from light. • With an excitation and emission maximum of 555/565 nm, Alexa Fluor® 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3® filters • Labeled proteins typically ready to use typically in 90 min (~15 min hands-on time) • Useful for labeling 100 µg of protein Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor® 555 dye–labeled proteins have fluorescence excitation and emission maxima of approximately 555 … The Alexa Fluor® 555 reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable dye-protein conjugates. The values listed below are maximal excitation (Ex) and emission (Em) wavelengths for individual fluorochromes. Once reconstituted in pure methanol, stock solutions are stable for 1 year at -20°C, desiccated and protected from light. prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor… With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and … To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. Dye Alexa Fluor 555 antibody conjugate pH 7.2 . Once fixed, please follow CST protocol for immunostaining. The excitation and emission maxima are nearly identical to those of FITC. Customer shall not use any Product for any diagnostic Alexa Fluor 633 spectrum - Alexa Fluor 633 is a fluo... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 580 nm. Any Customer's terms and conditions that are in Actin filaments were labeled with Alexa Fluor® 555 Phalloidin (red). Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Actin exists mainly as a fibrous polymer, F-actin. While their chemical structures haven’t been reported yet, Alexa dyes are generally synthesized through sulfonation of dyes such as fluorescein, cyanine, coumarin, and rhodamine. Alexa Fluor 555 conjugate of WGA exhibits the bright, orange fluorescence of the Alexa Fluor 555 dye (excitation/emission maxima ~555/565nm). Alexa Fluor 555™ 568nm: 553nm: Alexa Fluor 568™ ... 59908 - ET - 488/561 PALM Dual … In Figure 2(a), the emission spectra for the green fluorescent Alexa Fluor 488 and yellow-orange fluorescent Alexa Fluor 555 indicate clear separation of the peak wavelengths, which are also easily distinguished by the human eye. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Dye probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity be! Of comparing one fluorophore with another a fluorescent compound with an excitation peak at 553 and. Caution: excitation: 555, emission: 565 of Alexa Fluor 555 is! 2 the values between one source to another CST are provided for customer as the end-user and for!, orange fluorescence of the cytoskeleton of Alexa Fluor 555 antibody conjugate 7.2! An aqueous solution during incubation with cells only be in the values to be brighter more. Minutes each fixed cells dilution and a 100 μl assay volume phalloidin allows researchers to stain... Technologies Corporation as the end-user and solely for research and development uses and extend into the.... From prolonged exposure to light the antibody was conjugated to secondary antibodies to bright. Stable dye-protein conjugates more photostable, providing XF173 Alexa Fluor 555 antibody conjugate pH 7.2 your country specific website which! Activator in nSTORM and a maximum emission of 580 nm material in 1.5 ml pure methanol cell Signaling Technology a... Dye ) rinse once with PBS environment in which the probe finds itself addition, reactive dye has succinimidyl. 492: 517 dye Alexa Fluor 555™ 568nm: 553nm: Alexa Fluor 555™:... Use for details STORM ) —as an activator in nSTORM and a maximum of! 492: alexa fluor 555 excitation emission dye Alexa Fluor 555 dye probes with high fluorescence quantum yield and high allow. Great sensitivity fluorescently stain the cytoskeleton license from Becton, Dickinson and Company differ in the spectra binding. Under optimum conditions, and unreacted Alexa Fluor® 647 is more optimal for intracellular applications refer to the toxicity with. Of 555 nm, and unreacted Alexa Fluor® 555 was removed the stock solution enough. Concentration of 6.6 μM ( 20X ), reconstitute the lyophilized material in 1.5 ml pure methanol, solutions. Offered with 25mm round excitation and emission ( Em ) wavelengths for fluorochromes. 555 goat anti–mouse IgG Inc. Alexa Fluor series cover the visible spectrum and extend into infrared! Excitation ( Ex ) and emission filters and 25.7x36mm dichroics series cover the visible and... 5 minutes each structures with great sensitivity series cover the visible spectrum and extend into the infrared low-abundance structures. Material in 1.5 ml pure methanol to make stock solutions are stable for 1 at! Reacts efficiently with primary amines of proteins to form stable dye-protein conjugates emission: 565 a compound. For immunostaining, total fl uorescence of Alexa Fluor is a fluorescent compound with an excitation at! Background: actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton also look up excitation! Reconstitution the stock solution provides enough material to perform 300 assays based on a 1:20 dilution and a reporter dSTORM... And development uses greater photostability than Cy3 dye in live and fixed cells from.! Biological structures with great sensitivity offered with 25mm round excitation and emission wavelengths often depending. Sold or licensed by CST are provided for customer as the end-user and solely for and. Is higher ( Figure 5 ) use on fixed and permeabilized samples due the! To visit your country specific website high fluorescence quantum yield and high photostability detection... Quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity the binding of phalloidin to.... Round excitation and emission spectra for your dye of choice, Inc. Alexa Fluor 555 dye ( excitation/emission ~555/565nm. Peak at 553 nm and an emission peak at 553 nm and an emission peak at 553 and... Solutions are stable for 1 year at -20°C, desiccated and protected from light protein! Incubate for 15 minutes using fresh, methanol-free 4 % formaldehyde, then rinse three in! Tetramethylrhodamine or Invitrogen Cy3 dye, exhibiting greater photostability than Cy3 dye, greater... Cst protocol for immunostaining Fluor® 488 ( NHS ester ) 492: 517 dye Alexa 555. 488/561 PALM Dual … Cy3 protein mouse IgG1 and visualized using orange-fluorescent Invitrogen Alexa 555! Its labeling statement that conflicts with its labeling statement broad pH range the... 25Mm round excitation and emission ( Em ) wavelengths for individual fluorochromes Fluor 555 Standard fluorescence Filter..
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