For Research Use Only. No. Maximum blot size is 9 cm x 9 cm. Description This set contains a Mini Gel Tank for protein separation and two Mini Blot Modules for Western transfer. Watch this step by step video of how to run a NuPAGE precast get in the SureLock Tank. Proteins from the gel were transferred onto a 0.45 µm PVDF membranes at 25V constant for 30 minutes using the SureLock Tandem Midi Blot Module. The expected current listed in the table below is for transferring one gel. Total protein normalization of transferred proteins shows uniform transfer across the blot. The densitometric signal intensity was determined for each lane. Find the right set of products that can assist you with detection and analysis of your protein. We offer three systems for tank transfer that minimize the amount of transfer buffer required. To demonstrate the SureLock Tandem Midi Blot Module’s uniformity of transfer across a membrane, a NuPAGE 4-12% Bis-Tris Midi gel was loaded with recurring dilutions of E. coli lysate ranging from 2-0.25 µg and SeeBlue Plus2 Pre-stained Protein Standard. When used with high-performance Invitrogen precast midi gels, you can depend on the SureLock Tandem Midi Gel Tank and Midi Blot Module to generate consistent, high-throughput western blots. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high … Create Account. Uniform transfer using SureLock Tandem Midi Gel Tank. Figure 1. Download: Protein gel electrophoresis technical handbook, Download: Western Detection Workflow brochure, Protein Electrophoresis and Western Blotting Education Center. Wet Tank Transfer Systems Wet transfer is the most common and traditional strategy for western blot transfer due to its efficiency and throughput. When your western blotting workflow requires higher throughput, Invitrogen midi gels will allow you to load up to 26 samples per gel. Two SureLock Tandem Midi Blot Modules fit into the innovative, dual-purpose SureLock Tandem Midi Gel Tank and are designed for easy, room-temperature gel transfers. The image was captured using an iBright Imager with the No-Stain Membrane epi setting (455-485 nm excitation and 565-615 nm emission). Tris-Glycine Transfer Buffer with 20% methanol. For overnight blotting, transfer at a constant voltage of 10-15 V. Learn how to perform a wet tank transfer using the XCell II Blot Module in the SureLock Tank with this detailed step by step video. ZA10001). Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Bis-Tris transfer buffer with 10% methanol and antioxidant for reduced samples, SureLock Tandem Midi Pre-cut Membranes and Filters, 0.2 μm, Nitrocellulose. As the SeeBlue Plus2 Pre-stained Protein Standard is already labeled, its protein bands are not all susceptible to labeling with No-Stain Reagent, and therefore are not all visible in this image. After transfer, the proteins on the membrane were labeled following the No-Stain Protein Labeling Reagent protocol for midi-sized membranes. The gel was electrophoresed using MES running buffer in the SureLock Tandem Midi Gel Tank. Thermo Fisher Scientific. Only 300 mL of methanol-containing buffer is required per gel transfer, reducing buffer and hazardous disposal costs. The XCell II Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. A. No-Stain Reagent Labeled PVDF Blot. Technical replicates (n=4) were averaged and plotted to determine the linear regression for the entire concentration range (R2=0.9994). Transfer membranes Transfer buffers Error bars represent standard deviation.
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